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氟化物对FRTL细胞甲状腺功能因子代谢的影响 被引量:1

Effects of Fluoride on the Metabolism of Thyroid Function Factors in FRTL
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摘要 为了探讨氟化物对甲状腺功能因子代谢的影响,本实验传代培养FRTL细胞,用氟化钠处理对数生长期的细胞,使培养液中氟水平分别为0、1.25、2.5、5、10、20mg/L。分别于24和72h后收集细胞上清液,测定上清液中游离甲状腺激素FT3和FT4,甲状腺球蛋白(TG)以及甲状腺过氧化物酶(TPO)的活性。实验结果表明,NaF处理FRTL后,细胞上清液中FT3、FT4水平和TPO活性也不同程度降低,且与添加的氟化物浓度呈现显著的负相关(P【0.05);上清液中TG的含量除了NaF为2.5mg/L处理组比对照组有所升高外,其他浓度作用的FRLT上清液中TG含量均降低,整体上TG含量与氟化物浓度之间也呈现显著的负相关(P【0.05)。氟化物导致FRTL分泌TG不足,TPO活性及FT3、FT4水平下降,从而导致甲状腺功能降低。本实验揭示了氟化物对甲状腺细胞功能因子代谢的影响规律,在一定程度上也为揭示氟致甲状腺肿的机理奠定了基础。 To investigate the effects of fluoride on the metabolism of thyroid function factors,Fisher rat thyroid cell line(FRTL)in logarithmic growth phase were treated with 0,1.25,2.5,5,10,20 mg/L of sodium fluoride(NaF),respectively.The cell culture supernatant of FRTL was collected at 24 hand 72h.The contents of free thyroid hormones(FT3,FT4)and Thyroglobulin(TG)in the supernatant were determined as well as the activities of thyroid peroxidase(TPO)were detected.The results showed that,compared with the control,the contents of FT3,FT4 and the activities of TPO in the groups treated with NaF decreased.The TG content in supernatant of FRTL also decreased with an exception of treatment with2.5mg/L of NaF,in which it increased slightly.The levels of NaF significantly and negatively correlated with the contents of FT3,FT4,TG,and the activities of TPO,respectively(P<0.05).Fluoride restrained the function of thyroid cell due to decrease the levels of function factors FT3,FT4 and TG as well as the activities of TPO.The research is helpful for clarifying the metabolism of goiter caused by fluoride.
作者 刘国艳 江鹏 柴春彦 LIU Guo-yan;JIANG Peng;CHAI Chun-yan;School of Agriculture and Biology,Shanghai Jiaotong University,Key Lab of Shanghai Veterinary Bio-tech;
出处 《上海交通大学学报:农业科学版》 2014年第6期82-85,共4页 Journal of Shanghai Jiaotong University(Agricultural Science)
基金 国家自然基金资助项目(No.30671544)
关键词 氟化物 FRTL 甲状腺激素 甲状腺球蛋白 甲状腺过氧化物酶 fluoride FRTL thyroid hormones thyroglobulin thyroid peroxidase
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参考文献10

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